Western Blot

"Western blotting is a technique used to identify and locate proteins based on their ability to bind to specific antibodies."

Western Blot is what I had to do for this whole week.
The comfort bubble that I was in during undergrad burst so suddenly, that
I had no time to rebound....while I'm doing what I love, the lazy bone still stays in me.

To destroy and reconstruct is not as simple as it sounds to be. To be able to destroy requires
a willing heart to let go....
Breaking habits and creating new "good" ones XD

If science seems like a chore do you still do it?
Maybe it's just because I still lack of skill...
Always doing wrong calculations...in my brain..apparently my thoughts are failing me
and a piece of paper is required..I hope my Western turns out awesome tomorrow!

Can't wait!

New Year's Resolution

It's been a long time, really long...one month into the new year it's time to have my resolution written down. Things that are beneficial are never too late to do :p
The need to organize and revamp my lifestyle is almost essential, I need a template for my blog and my life otherwise consistency cannot be achieved, that's what I need.
So from now on this blog will just be about my day. Ja ikimashou!!

I woke up and turned on my laptop while browsing some useless videos, cooked rice, eggs. Went to take a shower, shaved a little, ate, and took interval naps of 5 mins.

Thought: Should have just practiced piano and ate. Waking up too early is useless if I just sit in front of my laptop.

Went to class, got a sandwich, went to piano class.

Thought: Sleepy in my NeuroSci class, dammit nicotine withdrawal!! Yeah need more concentration, and study anatomy more. Where the nerves cross, where to projections go etc.......feel left out; not good; need to study!!! Piano class was almost a reset, oh well I need it!

Came to lab, talked to Marcelo, got P1, dick around online again, talked to James about genotyping. Made NaOH buffer, Tris buffer took reallllly long to get to pH 5 (in 100 mL of tris 8.1, use 5 mL of concentrated HCl and then use 3 N HCl to slowly get there! lol) Cut tails to use a little bit. Wasted more time on laptop -.- Ok ........made reactions, then proceed to PCR.

Thought: What waste of time, need to fit in some reading in between waiting. Again write down what is needed for the experiment before hand, make sure everything is ready before doing it. Planning will save me a lot of time. Spin down the tissues before cooking to make sure they will dissolve!! Maybe have a timeline!

Heh that's about it, pretty boring IMHO but this will help me with my failing memory, and BTW 3rd day w/o smoking fuck yeah!!!

Tolerance and People

"To forgive is divine, to forget....impossible"

After 2 and a half years living in Chicago, I have seen people from all walks of life (well don't really care for the uber rich). There are people I wouldn't mind hanging out with and there are people that I would absolutely avoid.

Coming to Madison, I have made a stupid decision to look for an apartment late...well there are always lesson to be learned from tardiness. So I ended up in an awesome place (comes with a price), but unfortunately I have been paired up with one of those people that I would never even dreamed of living with.

I have always been a passive person (in terms of avoiding conflict), and this time I have to deal with all sorts of annoyance. I am a patient person, and I've come to learn that. Dealing with people is not my forte, I can only endure and bitch (which I should really stop doing). However, it is not like I did nothing, I tried my best talking to him and although I successfully abated the situation, the little nuances continue to irritate me. I often wonder if these things are just purely testing my tolerance or do I actually have to do something?

Lost? Am I?

The road to Spring Formal wasn't anything close to excitement but I ended up going anyway, picking up a couple of misunderstandings along the way. Of course, it's not what I wanted to do but heck things always never turn out in my favor.

At least the food was much better and I wish the venue was a tad bigger. Of course the dance floor. There was barely any breathing space and many of us had to cram or resolve to dancing by the sidelines.

It was overall a pretty fun experience except that I left with a tad of disappointment lingering in my still empty [insert organ]. Hopefully nothing changes after this Formal as it was all in the name of fun.

One comment though, these days guys can only grind. You can hardly see any real dancing any more, or is it just a club-culture we have so developed. Maybe I just want attention =p

Power to the people?

Recently, actually last Friday our dear ex-Prime Minister Pak Lah decided to step down from his position. This came as a shock to some of my friends while I was thinking "Finally?!". Obviously, because he didnt do much during his years as the PM and very well received tons of criticism. So, once again Malaysia outclasses itself by electing a murderer as our next PM well you-know-who right? I dont know which idiot prophesied R A H M A N as the sequence how our country's PM's were going to be lected but I think it's freaking retarded. Well it came true but at what cost? It was pretty awesome until M. So, who's gonna spell out the next sequence for our PMs-to-come? Or what is it? Ha!

Oh well, being a pure newbie or an ignorant fool of my country's politic...I'm not one to say anything. But I totally agree on the results of the elections held this and last year were the 'rakyat' begin to show what we are made of. I didnt vote (booo) but I'm sure the BNs will be more conscious on what they are doing. Vote them out if they do a shit job I say. If anyone care to update me on the situation now it would be awesome as I have noticed there are plenty of outrage going on in the web.

Past past past

I seemed that i still cant get rid of my old self...ugh...this is getting hard...maybe I have too much elemental mercury in my brain =p...oh well...one observation..I have been rather repetitive with my comments especially positive one: take care, good luck...and etc...so cliche...maybe I need to increase my vocabulary >.<

This Spring break is going to be a boring one....actually not really..just void of people that I usually hang out with..otherwise i would say it's the same...with more catching up to do..today was the first time I ran to the lake then subsequently to Adler Planeterium..it was awesome..the Azure sky was almost like a calling to me!! My only regret is that I didnt bring my SLR...going to next time!! Well there was a couple making out...definitely NSFW...with all the kids and all...gosh! (maybe I'm just jealous =p)

Otherwise today is just studying!! Again didn't get much done..but something is always better than nothing..rite?

Cornell, The time when Sam becomes a bum

Hi folks!!

Last Thursday saw the beginning of a painful interview weekend at Cornell for me. It was not much..I just didn't sleep last Wednesday night due to my dumb dumb flight time of 6 am....while waiting I chatted with a guy from Guatemala...haha....well I slept through most of my uneventful flight until Philly..where i noticed a bitch..i just chose to call her that =p

The plane to Itaca was the smallest i've ever flew in my life....it had propellers!! OMFG...i was scared shit..well i slept through it eventually...and omg Ithaca was gorgeous..that night we had dinner and there was this applicant who had DNA tattooed all over his arm..it was cool..but you know....I dont know if I can do that...

As usual they assigned me a roommate from China -.- so the next night I too experienced how Chinamen sleep in their undies...oh well...he's another married man lol...why are these people married so early? Oh wait he's...28.....Ok skipping through all that boring crap!! We then had dinner at Cornell's president's house!! How cool is that! It was like a catered private party with an acapella group singing as a finale! Woot! Way to lure in graduate students...After dinner we went drinking (as usual) to a local pub....then this graduate student brought us into a club full of ABCs....boy it was weird..and oh I met a Malaysian girl during the interview too...well she was brought up in the States...so doesnt count =p

And then come Sunday...when it was tres foggy....like fucking foggy...and so..my damn plane was delayed..til monday...thinking that I could put up a night in the airport..I proceed with doing my shit for the day until....midnight...so this guy came up and told me i need to leave cause of "security" reasons...he told me there was no staff so they dont let anyone stay overnight...and here i thought i could have the airport all by myself...pssh...and so....i went out with my luggage out in the middle of the night...with the rain...this is the culmination of my trip! The day I wore a bum's shoes..for 4 fucking hours!!! First i tried sleeping by the door step...I was paranoid with the wind blowing and all...and it also got colder..I gave up...then I picked up my laptop and watched some Balls of Steel for an hour till my battery died (Thanks Yar Ling!) It rained harder (gotta love mother nature)....so what the hell can I do? I pulled out my trusty poddy and started dancing....for like 1 and a half hour till my legs are sore and my eyes are droopy....1 hour to go! I was bored....and then I saw the dude in the airport! What a dick.....oh well....I then decided to pull out my Canon and started shooting....after sometime..I saw some strange creature grazing...they were 3 deers!! Aww how cute..I flashed at them (with my camera) and they hop hop hop away...i guess I was lucky that there wasn't a male in the group or else he would have stick his antlers into where the sun don't shine!! =p ....the clock hit 4 am and there i go back in the airport!!! Yippie!! This is the story of how i became a bum...for 4 hours...